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Implications of Animal Cloning Experiments for the Potential Cloning of Human Beings

James Robl, University Mass Amherst 

In vitro development of bovine nuclear transfer embryos using three different fibroblast cell lines

Treatment  Cleavage (%)  Blastocyst (%)
Control  936  585 (63)  178 (19)
CL1  3713  2271 (61)  377 (10)
CL2  233  125 (54)  21 (9)
CL3  672  495 (75)  30 (5)
BAF (adult fibrosis) 516  Na  43 (8)

Pregnancy results (40 days) after transfer of NT derived embryos using fetal and adult fibroblast

Treatments  Embryos 
Trans (2/cow) 
pregnant (%)
Fetuses Recovered
at 42-50 days (%)
Fetal fibroblasts  38  10 (42)  5 (31)
Adult fibroblast  18  5 (28)  0 (0)

In vivo development of nuclear transfer embryos (CL1 cell line)

Embryos per cow  Total
Total cows transferred  48
Open  30
Pregnant  18
% pregnant  38
Abortions  10
% Abortions  56
Abortions prior to 45 days  5
Abortions beyond 45 days  5
Calves to terms  8
Normal calves to term  7
% normal calves to term/cows transf.  15
% nomal calves to term/pregnant cows  39

Email link | Feedback | Contributed by: Boston University. Video adapted from the Issues for the Millennium Workshop

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