Implications of Animal <!g>Cloning Experiments for the Potential Cloning of Human Beings
<!g>James Robl, University Mass Amherst
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<!g>In vitro development of <!g>bovine nuclear transfer embryos
using three different fibroblast cell lines
| Treatment |
n |
Cleavage (%) |
<!g>Blastocyst (%) |
| Control |
936 |
585 (63) |
178 (19) |
| CL1 |
3713 |
2271 (61) |
377 (10) |
| CL2 |
233 |
125 (54) |
21 (9) |
| CL3 |
672 |
495 (75) |
30 (5) |
| BAF (adult fibrosis) |
516 |
Na |
43 (8) |
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Pregnancy results (40 days) after transfer of NT derived
embryos using fetal and adult fibroblast
| Treatments |
Embryos
Trans (2/cow) |
Cows
pregnant (%) |
Fetuses Recovered
at 42-50 days (%) |
| Fetal <!g>fibroblasts |
38 |
10 (42) |
5 (31) |
| Adult fibroblast |
18 |
5 (28) |
0 (0) |
|
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<!g>In vivo development of nuclear transfer embryos (CL1 cell
line)
| Embryos per cow |
Total |
| Total cows transferred |
48 |
| Open |
30 |
| Pregnant |
18 |
| % pregnant |
38 |
| Abortions |
10 |
| % Abortions |
56 |
| Abortions prior to 45 days |
5 |
| Abortions beyond 45 days |
5 |
| Calves to terms |
8 |
| Normal calves to term |
7 |
| % normal calves to term/cows transf. |
15 |
| % nomal calves to term/pregnant cows |
39 |
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Email
link | Feedback | Contributed by: Boston University. Video adapted from the
Issues for the Millennium Workshop
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